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KMID : 0545120080180111803
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Journal of Microbiology and Biotechnology
2008 Volume.18 No. 11 p.1803 ~ p.1808
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Effect of Glutaraldehyde Treatment on Stability of Permeabilized Ochrobactrum anthropi SY509 in Nitrate Removal
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Park Young-Tae
Park Jae-Yeon
Park Kyung-Moon
Choi Suk-Soon
Yoo Young-Je
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Abstract
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For practical application, the stability of permeabilized Ochrobactrum anthropi SY509 needs to be increased, as its half-life of enzymatic denitrification is only 90 days. As the cells become viable after permeabilization treatment, this can cause decreased activity in a long-term operation and induce breakage of the immobilization matrix. However, the organic solvent concentration causing zero cell viability was 50%, which is too high for industrial application. Thus, wholecell immobilization using glutaraldehyde was performed, and 0.1% (v/v) glutaraldehyde was determined as the optimum concentration to maintain activity and increase the half-life. It was also found that 0.1% (v/v) glutaraldehyde reacted with 41.9% of the total amine residues on the surface of the cells during the treatment. As a result, the half-life of the permeabilized cells was increased from 90 to 210 days by glutaraldehyde treatment after permeabilization, and no cell viability was detected.
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KEYWORD
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Denitrification, permeabilization, glutaraldehyde, stability, immobilization
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